منابع مشابه
Method for electroporation for the early chick embryo.
In vitro whole-embryo culture of chick embryos, originally invented by New, has been widely used for studies of early embryogenesis. Here, a method for electroporation using the New culture and its derivatives is described, to achieve misexpression of exogenous gene in a temporally and spatially controlled manner in gastrulating chick embryos. Detailed information for the devices and procedures...
متن کاملEffects of monosodium glutamate on chick embryo retina in culture.
Monosodium glutamate added to 12-day chick embryo retinas in culture causes severe morphologic damage to the retina as judged by light microscopic examination. Damage is evident after a few hours with concentrations as low as 0.3 mM. Glutamyltransferase induction is also appreciably inhibited by the amino acid. General protein synthesis and RNA synthesis appear to be less affected.
متن کاملDifferentiation of chick embryo somites in chorioallantoic culture.
An analysis was made of the differentiative capabilities of somites isolated from all regions of stage-8 (4-somite) to stage-18 (36-somite) chick embryos, with particular reference to the incidence of spontaneous chondrogenesis. A chorioallantoic grafting technique with Millipore filter as a graft vehicle was employed. The youngest somites which spontaneously differentiated cartilage were the a...
متن کاملElectroporation of the hindbrain to trace axonal trajectories and synaptic targets in the chick embryo.
Electroporation of the chick embryonic neural tube has many advantages such as being quick and efficient for the expression of foreign genes into neuronal cells. In this manuscript we provide a method that demonstrates uniquely how to electroporate DNA into the avian hindbrain at E2.75 in order to specifically label a subset of neuronal progenitors, and how to follow their axonal projections an...
متن کاملIn vivo comparative study of RNAi methodologies by in ovo electroporation in the chick embryo.
The combination of emergent RNA interference (RNAi) technology with in ovo electroporation in the chick embryo has the potential to provide a powerful and rapid means for functional analyses of novel genes in vivo. In this study, we show that electroporation of short 21-bp RNA duplexes (siRNAs) is a quick and simple method for silencing exogenous and endogenous gene expression in vivo. Quantita...
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ژورنال
عنوان ژورنال: Current Protocols in Cell Biology
سال: 2012
ISSN: 1934-2500,1934-2616
DOI: 10.1002/0471143030.cb1915s56